Lampiran A. Surat Rekomendasi Kode Etik Penelitian Kesehatan

Please download to get full document.

View again

of 27
2 views
PDF
All materials on our website are shared by users. If you have any questions about copyright issues, please report us to resolve them. We are always happy to assist you.
Document Description
49 Lampiran A. Surat Rekomendasi Kode Etik Penelitian Kesehatan 50 Lampiran B. Tabel Konversi Perhitungan Dosis untuk Berbagai Jenis Hewan dan Manusia (berdasarkan luas permukaan tubuh) Mencit 20 g Tikus
Document Share
Document Transcript
49 Lampiran A. Surat Rekomendasi Kode Etik Penelitian Kesehatan 50 Lampiran B. Tabel Konversi Perhitungan Dosis untuk Berbagai Jenis Hewan dan Manusia (berdasarkan luas permukaan tubuh) Mencit 20 g Tikus 200g Marmut 400 g Kelinci 2 kg Kucing 2 kg Kera 4 Kg Anjing 12 Kg Manusia 70 Kg Mencit Tikus Marmut Kelinci Kucing Kera Anjing Manusia 20 g 200g 400 g 2 kg 2 kg 4 Kg 12 Kg 70 Kg 1,0 7,0 12,25 27,8 29,7 64,1 124,2 387,9 0,14 1,0 1, ,2 9,2 17,8 56,0 0,08 0,57 1,0 2,25 2,4 5,2 10,2 31,5 0,04 0,25 0,44 1,0 1,08 2,4 4,5 14,2 0,03 0,23 0,41 0,92 1,0 2,2 4,1 13,0 0,016 0,11 0,19 0,42 0,45 1,0 1,9 6,1 0,008 0,06 0,10 0,22 0,24 0,52 1,0 3,1 0,0036 0,018 0,031 0,07 0,076 0,16 0,32 1,0 Sumber : Percobaan Hewan Laboratorium (Soehardjono, 1993 dalam Wibowo, 2009) 51 Lampiran C. Penentuan Dosis Aloksan Pada peneliatian ini dengan menggunakan metode Etuk (2009), yaitu dengan menggunakan aloksan dengan dosis 180 mg/kg bb pada tikus untuk menginduksi diabetes melitus. Faktor konversi dosis untuk tikus dengan berat badan 200 g pada mencit dengan berat badan 20 g adalah 0,14. Dosis untuk mencit dengan berat 20 g adalah = = = 36 mg x 0,14 = 5,04 mg 52 Lampiran D. Penentuan Dosis Ekstrak Pada penelitian ini dengan menggunakan metode Lumban Raja (2009), yaitu dengan menggunakan ekstrak etanol biji mahoni dengan dosis bertingkat, yaitu 50mg/kg bb, 100mg/kg bb, 150mg/kg bb yang diberikan pada tikus. Faktor konversi dosis untuk tikus dengan berat badan 200 g, pada mencit dengan berat badan 20 g adalah 0,14. Dosis 1 untuk mencit dengan 20 g = 1,4 mg = = =10 mgx0,14 =1,4 mg Dosis 2 untuk mencit dengan 20 g = 2,8 mg = = =20 mgx0,14 =2,8 mg Dosis 3 untuk mencit dengan 20 g = 4,2 mg = = =30 mgx0,14 =4,2 mg 53 Lampiran E. Volume maksimum larutan sediaan uji yang dapat diberikan pada beberapa spesies hewan coba (ml) Intravena Intramuscular Intraperitoneal Sub cutan Per oral Mencit (20-30g) 0,5 0,05 1,0 1,5-1,0 1,0 Tikus (100g) 1,0 0,1 2,5 2,5 5,0 Hamster (50g) - 0, ,5 2,5 Marmot (250g) - 0, ,0 10,0 Merpati (300g) 2,0 0,5 2,0 2,0 10,0 Kelinci (2,5kg) , ,0 Kucing (3kg) , ,0 Anjing (5kg) , ,0 100,0 Sumber: (Ritschel,1974 dalam Sulastry, 2009)) 54 Lampiran F. Data Mentah Hasil Pengamatan No. Perlakuan Berat Badan (gram) KGD Puasa (mg/dl) Hari ke 1 KGD Diabetes (mg/dl) Hari ke 2 KGD Akhir (mg/dl) Hari ke 3 Jumlah Sel Raksasa 1 KNU1 29, KNU2 33, KNU3 35, KNU4 32, KNU5 27, KPU1 30, KPU2 29, KPU3 33, KPU4 35, KPU5 32, P1U1 30, P1U2 31, P1U3 28, P1U4 29, P1U5 33, P2U1 29, P2U2 31, P2U3 26, P2U4 32, P2U5 28, P3U1 26, P3U2 32, P3U3 28, 55 24 P3U4 34, P3U5 22, Lampiran G. Analisis Statistik Rerata Kadar gula Darah Tests of Normality Kolmogorov-Smirnov(a) Shapiro-Wilk Perlakuan Statistic Df Sig. Statistic Df Sig. KGD_H_1 KN,200 5,200(*),971 5,879 KP,200 5,200(*),920 5,531 P1,249 5,200(*),852 5,200 P2,264 5,200(*),946 5,710 P3,254 5,200(*),883 5,322 * This is a lower bound of the true significance. a Lilliefors Significance Correction Test of Homogeneity of Variance Levene Statistic df1 df2 Sig. KGD_H_1 Based on Mean, ,576 KGD_H_1 Based on Median, ,734 Based on Median and with adjusted df, ,222,735 Based on trimmed mean, ,572 ANOVA Sum of Squares Df Mean Square F Sig. Between Groups 11665, ,260 6,759,001 Within Groups 8629, ,460 Total 20294,240 24 56 Dependent Variable: KGD_H_1 Bonferroni (I) Perlakuan (J) Perlakuan Multiple Comparisons Mean Difference (I- J) Std. Error Sig. 95% Confidence Interval Lower Bound Upper Bound Lower Bound Upper Bound Lower Bound KN KP 52,20000(*) 13,13712,007 10, ,6266 P1 57,40000(*) 13,13712,003 15, ,8266 P2 35, ,13712,135-5, ,0266 P3 56,20000(*) 13,13712,004 14, ,6266 KP KN -52,20000(*) 13,13712,007-93, ,7734 P1 5, , ,000-36, ,6266 P2-16, , ,000-58, ,8266 P3 4, , ,000-37, ,4266 P1 KN -57,40000(*) 13,13712,003-98, ,9734 KP -5, , ,000-46, ,2266 P2-21, , ,000-63, ,6266 P3-1, , ,000-42, ,2266 P2 KN -35, ,13712,135-77,0266 5,8266 KP 16, , ,000-24, ,0266 P1 21, , ,000-19, ,2266 P3 20, , ,000-20, ,0266 P3 KN -56,20000(*) 13,13712,004-97, ,7734 KP -4, , ,000-45, ,4266 P1 1, , ,000-40, ,6266 P2-20, , ,000-62, ,8266 * The mean difference is significant at the.05 level. 1. Lampiran Data Statistik KGD hari ke-4 Tests of Normality Kolmogorov-Smirnov(a) Shapiro-Wilk Perlakuan Statistic Df Sig. Statistic df Sig. KGD_H_2 KN,351 5,044,728 5,018 KP,363 5,030,787 5,063 P1,284 5,200(*),824 5,125 P2,420 5,004,642 5,002 P3,178 5,200(*),930 5,597 * This is a lower bound of the true significance. a Lilliefors Significance Correction 57 Test of Homogeneity of Variance Levene Statistic df1 df2 Sig. KGD_H_2 Based on Mean 3, ,020 Based on Median, ,571 Based on Median and with adjusted df, ,267,598 Based on trimmed mean 2, ,055 Tests of Normality Kolmogorov-Smirnov(a) Shapiro-Wilk Perlakuan Statistic Df Sig. Statistic df Sig. KGD_H_2 KN,351 5,044,728 5,018 KP,363 5,030,787 5,063 P1,284 5,200(*),824 5,125 P2,420 5,004,642 5,002 P3,178 5,200(*),930 5,597 * This is a lower bound of the true significance. a Lilliefors Significance Correction Test of Homogeneity of Variance Levene Statistic df1 df2 Sig. KGD_H_2 Based on Mean 1, ,196 Based on Median, ,702 Based on Median and with adjusted df, ,418,705 Based on trimmed mean 1, ,286 Kruskal-Wallis Test Perlakuan N Mean Rank KGD_H_2 KN 5 3,80 KP 5 17,90 P1 5 9,10 P2 5 15,30 P3 5 18,90 Total 25 Test Statistics(a,b) KGD_H_2 Chi-Square 15,146 58 Df 4 Asymp. Sig.,004 a Kruskal Wallis Test Mann-Whitney Test Perlakuan N Mean Rank Sum of KGD_H_2 KN 5 3,00 15,00 KP 5 8,00 40,00 KGD_H_2 Mann-Whitney U,000 Wilcoxon W 15,000 Z -2,611 Asymp. Sig. (2-tailed),009,008(a) Perlakuan N Mean Rank Sum of KGD_H_2 KN 5 3,60 18,00 P1 5 7,40 37,00 KGD_H_2 Mann-Whitney U 3,000 Wilcoxon W 18,000 Z -1,984 Asymp. Sig. (2-tailed),047,056(a) Perlakuan N Mean Rank Sum of KGD_H_2 KN 5 3,20 16,00 P2 5 7,80 39,00 59 KGD_H_2 Mann-Whitney U 1,000 Wilcoxon W 16,000 Z -2,402 Asymp. Sig. (2-tailed),016,016(a) Perlakuan N Mean Rank Sum of KGD_H_2 KN 5 3,00 15,00 P3 5 8,00 40,00 KGD_H_2 Mann-Whitney U,000 Wilcoxon W 15,000 Z -2,611 Asymp. Sig. (2-tailed),009,008(a) Perlakuan N Mean Rank Sum of KGD_H_2 KP 5 7,80 39,00 P1 5 3,20 16,00 KGD_H_2 Mann-Whitney U 1,000 Wilcoxon W 16,000 Z -2,402 Asymp. Sig. (2-tailed),016,016(a) 60 Perlakuan N Mean Rank Sum of KGD_H_2 KP 5 6,00 30,00 P2 5 5,00 25,00 KGD_H_2 Mann-Whitney U 10,000 Wilcoxon W 25,000 Z -,522 Asymp. Sig. (2-tailed),602,690(a) Perlakuan N Mean Rank Sum of KGD_H_4 KP 5 5,10 25,50 P3 5 5,90 29,50 KGD_H_4 Mann-Whitney U 10,500 Wilcoxon W 25,500 Z -,419 Asymp. Sig. (2-tailed),675,690(a) Perlakuan N Mean Rank Sum of KGD_H_2 P1 5 4,30 21,50 P2 5 6,70 33,50 KGD_H_2 Mann-Whitney U 6,500 Wilcoxon W 21,500 Z -1,257 Asymp. Sig. (2-tailed),209 61,222(a) Perlakuan N Mean Rank Sum of KGD_H_2 P1 5 3,20 16,00 P3 5 7,80 39,00 KGD_H_2 Mann-Whitney U 1,000 Wilcoxon W 16,000 Z -2,402 Asymp. Sig. (2-tailed),016,016(a) Perlakuan N Mean Rank Sum of KGD_H_2 P2 5 4,80 24,00 P3 5 6,20 31,00 KGD_H_2 Mann-Whitney U 9,000 Wilcoxon W 24,000 Z -,731 Asymp. Sig. (2-tailed),465,548(a) Tests of Normality Kolmogorov-Smirnov(a) Shapiro-Wilk Perlakuan Statistic df Sig. Statistic df Sig. KGD_H_16 KN,203 5,200(*),976 5,914 KP,256 5,200(*),952 5,753 P1,294 5,184,781 5,057 P2,405 5,007,661 5,004 62 P3,200 5,200(*),950 5,734 * This is a lower bound of the true significance. a Lilliefors Significance Correction Test of Homogeneity of Variance Levene Statistic df1 df2 Sig. KGD_H_16 Based on Mean 4, ,012 Based on Median, ,489 Based on Median and with adjusted df, ,701,536 Based on trimmed mean 3, ,037 Tests of Normality Kolmogorov-Smirnov(a) Shapiro-Wilk Perlakuan Statistic df Sig. Statistic df Sig. KGD_H_16 KN,203 5,200(*),976 5,914 KP,256 5,200(*),952 5,753 P1,294 5,184,781 5,057 P2,405 5,007,661 5,004 P3,200 5,200(*),950 5,734 * This is a lower bound of the true significance. a Lilliefors Significance Correction Test of Homogeneity of Variance Levene Statistic df1 df2 Sig. KGD_H_16 Based on Mean 3, ,042 Based on Median, ,467 Based on Median and with adjusted df, ,395,511 Based on trimmed mean 2, ,090 Kruskal-Wallis Test Perlakuan N Mean Rank KGD_H_16 KN 5 3,90 KP 5 21,80 P1 5 15,10 P2 5 12,60 P3 5 11,60 Total 25 Test Statistics(a,b) KGD_H_16 63 Chi-Square 15,407 Df 4 Asymp. Sig.,004 a Kruskal Wallis Test Mann-Whitney Test Perlakuan N Mean Rank Sum of KGD_H_16 KN 5 3,00 15,00 KP 5 8,00 40,00 KGD_H_16 Mann-Whitney U,000 Wilcoxon W 15,000 Z -2,611 Asymp. Sig. (2-tailed),009,008(a) Perlakuan N Mean Rank Sum of KGD_H_16 KN 5 3,00 15,00 P1 5 8,00 40,00 KGD_H_16 Mann-Whitney U,000 Wilcoxon W 15,000 Z -2,611 Asymp. Sig. (2-tailed),009,008(a) Perlakuan N Mean Rank Sum of KGD_H_16 KN 5 3,60 18,00 P2 5 7,40 37,00 64 KGD_H_16 Mann-Whitney U 3,000 Wilcoxon W 18,000 Z -1,984 Asymp. Sig. (2-tailed),047,056(a) Perlakuan N Mean Rank Sum of KGD_H_16 KN 5 3,30 16,50 P3 5 7,70 38,50 KGD_H_16 Mann-Whitney U 1,500 Wilcoxon W 16,500 Z -2,305 Asymp. Sig. (2-tailed),021,016(a) Perlakuan N Mean Rank Sum of KGD_H_16 KP 5 7,80 39,00 P1 5 3,20 16,00 KGD_H_16 Mann-Whitney U 1,000 Wilcoxon W 16,000 Z -2,402 Asymp. Sig. (2-tailed),016,016(a) 65 Perlakuan N Mean Rank Sum of KGD_H_16 KP 5 7,00 35,00 P2 5 4,00 20,00 KGD_H_16 Mann-Whitney U 5,000 Wilcoxon W 20,000 Z -1,567 Asymp. Sig. (2-tailed),117,151(a) Perlakuan N Mean Rank Sum of KGD_H_16 KP 5 8,00 40,00 P3 5 3,00 15,00 KGD_H_16 Mann-Whitney U,000 Wilcoxon W 15,000 Z -2,611 Asymp. Sig. (2-tailed),009,008(a) Perlakuan N Mean Rank Sum of KGD_H_16 P1 5 6,40 32,00 P2 5 4,60 23,00 66 KGD_H_16 Mann-Whitney U 8,000 Wilcoxon W 23,000 Z -,940 Asymp. Sig. (2-tailed),347,421(a) Perlakuan N Mean Rank Sum of KGD_H_16 P1 5 6,50 32,50 P3 5 4,50 22,50 KGD_H_16 Mann-Whitney U 7,500 Wilcoxon W 22,500 Z -1,048 Asymp. Sig. (2-tailed),295,310(a) Perlakuan N Mean Rank Sum of KGD_H_16 P2 5 5,60 28,00 P3 5 5,40 27,00 KGD_H_16 Mann-Whitney U 12,000 Wilcoxon W 27,000 Z -,104 Asymp. Sig. (2-tailed),917 1,000(a) 67 Lampiran H. Analisis Statistik Rerata Berat Badan Tests of Normality Kolmogorov-Smirnov(a) Shapiro-Wilk Perlakuan Statistic df Sig. Statistic df Sig. Berat_badan KN,204 5,200(*),969 5,867 KP,155 5,200(*),990 5,980 P1,137 5,200(*),990 5,981 P2,127 5,200(*),992 5,987 P3,197 5,200(*),954 5,764 * This is a lower bound of the true significance. a Lilliefors Significance Correction Test of Homogeneity of Variance Levene Statistic df1 df2 Sig. Berat_badan Based on Mean 1, ,161 Based on Median 1, ,368 Based on Median and with adjusted df 1, ,449,381 Based on trimmed mean 1, ,156 Berat_badan ANOVA Sum of Squares Df Mean Square F Sig. Between Groups 38, ,725,967,447 Within Groups 201, ,060 Total 240,106 24 68 Lampiran I. Analisis Statistik Tingkat Perbandingan Jumlah sel Raksasa NPar Tests Kruskal-Wallis Test Perlakuan N Mean Rank Jumlah_Giant_Cell KN 5 4,50 KP 5 13,50 P1 5 15,70 P2 5 15,60 P3 5 15,70 Total 25 Test Statistics(a,b) Jumlah_Giant_ Cell Chi-Square 8,784 Df 4 Asymp. Sig.,067 a Kruskal Wallis Test NPar Tests Mann-Whitney Test Perlakuan N Mean Rank Sum of Jumlah_Giant_Cell KN 5 4,10 20,50 KP 5 6,90 34,50 Jumlah_Giant_ Cell Mann-Whitney U 5,500 Wilcoxon W 20,500 Z -1,514 Asymp. Sig. (2-tailed),130,151(a) 69 NPar Tests Mann-Whitney Test Perlakuan N Mean Rank Sum of Jumlah_Giant_Cell KN 5 3,00 15,00 P1 5 8,00 40,00 Jumlah_Giant_ Cell Mann-Whitney U,000 Wilcoxon W 15,000 Z -2,652 Asymp. Sig. (2-tailed),008,008(a) NPar Tests Mann-Whitney Test Perlakuan N Mean Rank Sum of Jumlah_Giant_Cell KN 5 3,40 17,00 P2 5 7,60 38,00 Jumlah_Giant_ Cell Mann-Whitney U 2,000 Wilcoxon W 17,000 Z -2,227 Asymp. Sig. (2-tailed),026,032(a) NPar Tests Mann-Whitney Test Perlakuan N Mean Rank Sum of Jumlah_Giant_Cell KN 5 3,00 15,00 70 P3 5 8,00 40,00 Jumlah_Giant_ Cell Mann-Whitney U,000 Wilcoxon W 15,000 Z -2,660 Asymp. Sig. (2-tailed),008,008(a) NPar Tests Mann-Whitney Test Perlakuan N Mean Rank Sum of Jumlah_Giant_Cell KP 5 5,30 26,50 P1 5 5,70 28,50 Jumlah_Giant_ Cell Mann-Whitney U 11,500 Wilcoxon W 26,500 Z -,210 Asymp. Sig. (2-tailed),834,841(a) NPar Tests Mann-Whitney Test Perlakuan N Mean Rank Sum of Jumlah_Giant_Cell KP 5 5,10 25,50 P2 5 5,90 29,50 Jumlah_Giant_ Cell Mann-Whitney U 10,500 Wilcoxon W 25,500 Z -,419 Asymp. Sig. (2-tailed),675 71 NPar Tests Mann-Whitney Test,690(a) Perlakuan N Mean Rank Sum of Jumlah_Giant_Cell KP 5 5,20 26,00 P3 5 5,80 29,00 Jumlah_Giant_ Cell Mann-Whitney U 11,000 Wilcoxon W 26,000 Z -,317 Asymp. Sig. (2-tailed),751,841(a) NPar Tests Mann-Whitney Test Perlakuan N Mean Rank Sum of Jumlah_Giant_Cell P1 5 5,40 27,00 P2 5 5,60 28,00 Jumlah_Giant_ Cell Mann-Whitney U 12,000 Wilcoxon W 27,000 Z -,105 Asymp. Sig. (2-tailed),916 1,000(a) NPar Tests Mann-Whitney Test Perlakuan N Mean Rank Sum of Jumlah_Giant_Cell P1 5 5,60 28,00 P3 5 5,40 27,00 72 Jumlah_Giant_ Cell Mann-Whitney U 12,000 Wilcoxon W 27,000 Z -,106 Asymp. Sig. (2-tailed),915 1,000(a) NPar Tests Mann-Whitney Test Perlakuan N Mean Rank Sum of Jumlah_Giant_Cell P2 5 5,50 27,50 P3 5 5,50 27,50 Jumlah_Giant_ Cell Mann-Whitney U 12,500 Wilcoxon W 27,500 Z,000 Asymp. Sig. (2-tailed) 1,000 1,000(a) 73 Lampiran J. Alur Penelitian Pemeliharaan mencit Puasa ± 16 Diberi diet standar Induksi aloksan 180 mg/kg BB Induksi aloksan 180 mg/kg BB + ekstrak etanol biji mahoni dosis 50 mg/kg BB Induksi aloksan 180 mg/kg BB + ekstrak etanol biji mahoni dosis 100 mg/kg BB Induksi aloksan 180 mg/kg BB + ekstrak etanol biji mahoni dosis 150 mg/kg BB Pengukuran Kadar Gula darah Pengukuran Berat badan Histologi limpa mencit 74 Lampiran K. Prosedur Pengolahan Ekstrak Biji Mahoni Biji mahoni Dibersihkan dari kulit pembungkusnya Dikeringkan tidak pada sinar matahari secara langsung Dihaluskan Serbuk biji mahoni Lampiran L. Prosedur Ekstrak Etanol Biji Mahoni Serbuk biji mahoni Maserat Dimasukkan ke dalam botol berwarna gelap Ditambahkan pelarut etanol 96% Ditutup dan dibiarkan selama dua hari terlindung dari cahaya Disaring Ekstrak Etanol biji mahoni Diuapkan menggunakan vakum putar pada suhu 40 0 C Dikeringkan Lampiran M. Prosedur Pembuatan Suspensi Ekstrak Etanol Biji Mahoni 0,5 g CMC Gel CMC Ekstrak etanol biji mahoni Larutan ekstrak Ditaburkan dalam lumpang yang berisi aquades panas 10 ml Didiamkan 15 menit Digerus hingga berbentuk gel Ditambahkan gel CMC sedikit demi sedikit Digerus sampai terbentuk suspensi Dicukupkan volumenya sampai 100 ml dengan akuades 75 Lampiran N. Prosedur Pembuatan Preparat Histologis Limpa Organ Limpa Difiksasi selama 1 hari Dicuci dengan alkohol 70% Didehidrasi dengan alkohol bertingkat Dijernihkan dengan Xylol Diinfiltrasi Ditanam dalam kotak kecil yang berisi parafin Dipotong pita parafin dengan menggunakan mikrotom Direndam dalam air dingin Direndam dalam air hangat Ditempelkan pada objek gelas Direkatkan dengan pemanasan di atas hot plate Dideparafinasi Diwarnai Ditutup dengan Canada balsam Preparat
We Need Your Support
Thank you for visiting our website and your interest in our free products and services. We are nonprofit website to share and download documents. To the running of this website, we need your help to support us.

Thanks to everyone for your continued support.

No, Thanks