Effect of electrochemical potential on a Shewanella Oneidensis MR 1 biofilm on the biosynthesis of Silver Nanoparticles

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Effect of electrochemical potential on a Shewanella Oneidensis MR 1 biofilm on the biosynthesis of Silver Nanoparticles
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  Silver nanoparticles (AgNPs) have been demonstrated to have greater antimicrobial activity thantheir silver salt counterparts due to the higher surface to area volume ratio. Conventional methodsused to produce nanoparticles due to the use of potentially hazardous compounds and/or extremesynthesis conditions raise concerns about their toxicity and environmental impact.Microbes are known for their bio-mineralisation ability. Biological synthesis of nano-materials is attracting much research interest due to low cost of the catalyst and low environmentalimpact. Furthermore biosynthetic mechanisms might result in efficient at producing nanoparticles of¬†the desired shape, size and distribution given the highly specific interactions of the bio moleculartemplates and inorganic materials. In turn, this is an advantage due to the size and shape dependantproperties of nanoparticles, however the full control of NPs shape and size in biosynthetic NPs is farfrom being achieved. Shewanella are dissimilar metal reducing bacteria, which forms electroactive biofilms, whichcan reduce metals via outer membrane cytochromes. We investigated the effect of varying thepotential applied to a Shewanella oneidenesis MR-1 biofilm, grown in a bio-electrochemical reactor,on size distribution of the resulting nanoparticles. We hypothesise that controlling the potentialapplied to the biofilm will allow great control of the transfer of electrons from the cytochrome tosilver ions, and will allow for a greater control of the size distribution.
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