234 Pretreatment with monoclonal antiidiotypic antibody (aId mAb) bearing the internal image of Lol p I. Effects on the anti-Lol p I responses in mice

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234 Pretreatment with monoclonal antiidiotypic antibody (aId mAb) bearing the internal image of Lol p I. Effects on the anti-Lol p I responses in mice
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  VOLUME 87 NUMBER 1. PART 2 Abstracts 197 233 COMPARISON F IMMlJNOBLOT PATTERNS BETWEEN LLERGIC BRONCHOPULMONARY ASPERGILLOSIS & ASPERGILLOMA. AH Wolff.MD. CL Holland.MD and 4 Bielorv.MD, East Orange, N.J. We compared the response of Aspergillus fumigatus (Af) specific IgG in Allergic Bronchopulmonary Aspergillosis (ABPA) and aspergilloma. Five patients (pts) were studied, 3 with ABPA and 2 with aspergilloma. Using the western blot technique (12 SDS-PAGE), we identified the binding pattern of Af specific IgG antibodies (Abs) to the recognized Af dominant antigens (Ags), i.e. 18,23-24,36- 38,40,52,55,63,70 and 80 kD. There was a mean of 13.0 bands in the aspergilloma pts vs 8.3 in ABPA. Of the 9 predominant antigenic bands for Af, the aspergilloma pts bound a mean of 7.5 vs 5.0 for ABPA. The aspergilloma pts had more bands to Ags ~41 kD (mean=7.0 vs 4.0 for ABPA), although the mean number of bands to antigens >41 kD was similar in both groups (4.3 for aspergilloma, 5.5 for ABPA). Thus in our aspergilloma pts, the IgG response to Af was more varied since a greater number of Af specific ags were bound than in the ABPA pts. Additionally, the abs response to the lower molecular weight Af antigens (~41 kD) was stronger in the pts with aspergilloma than with ABPA. 234 PRETREATMENT WITH MONOCLONAL ANTI- IDIOTYPIC ANTIBODY (aId mAb) BEARING THE INTERNAL IMAGE OF Lo1 p I. EFFECI’S ON THE ANTI- Lo1 p I RESPONSES N MICE. Y. B.Sc.. M.D. C.H.U.L. Qu&ec, Canada. In order to study the regulation of immune response to Lo1 p I, we have produced mAb against allergenic determinants of Lo1 p I and an aId mAb directed against one of these anti-Lo1 p I (29OA167) mAb. This aId mAb (A7H2) showed internal image properties of Lo1 p I and it could also inhibit the binding of IgE from allergic subjects to antigen and induce histamine release from basophils. The effects of pretreatmenti with A7H2 in BALB/c mice before immunization with the antigen have been studied. The aId mAb (0.1 or 0.01 mg) or unrelated mAb (control) were weekly given for 8 weeks (i.p). Then mice received the antigen (2 pg) adsorbed with alum (2 mg) at week 9,11,13 and 15. Serum anti-lo1 p I IgG, IgE Ab (direct binding) and specific Id (binding-inhibition of 29OA167 Id to Lo1 p I) responses were measured. Anti-Lo1 p I IgG could be detected before immunization with Lo1 p I (week 8) only in mice pretreated with aId mAb. Immunization with Lo1 p I induced an anti-Lo1 p I IgG response in both groups, but this response was significantly higher (p<O.O5) in mice that received aId mAh. Anti-la1 p I IgG response 09. at 406 nm &ELM.) Week aId mAb pretreated control aId {” 0.087 + 0.008 0.043 * 0.006 mAb8 0.626 0.147 0.021* 0.002 0.888 f 0.119 0.021 0.002 1.555 0.137 0.518 f 0.124 \l5 1.855 0.020 1.113f 0.188 Similar profiles were seen for IKE Ab and Id specific responses. -This study confirms-the internal image properties of A7H2 since it induced an anti-Lolp I response prior to sensitization with the antigen. 235 HUMAN T CELL RESPONSE O PURIFIED POLLEN ALLERGENS FTHE GRASS, OLWMPERENNE. S.Baskar, uti A.A. Ansari. Phs. Baltimore, MD. The aim of this study was to investigate T cell response o grass pollen allergens, Lol p I, Lol p II and Lol p III in non-atopic individuals, grass allergic individuals who were m on immunotherapy and grass allergic individuals who were on immunotherapy. en random volunteers rom each goup were studied and heir peripheral blood mononuclear ells (PBMC) were cultured n the absence r presence f different doses of ptiiicd antigens. People allergic to grasses (skin test positive) showed significant T cell proliferative response o Lo/p I and LOI p III. By contrast, people who were allergic to grasses nd were receiving immunotherapy with grass mixtures showed comparatively ower T cell responses o LOI p I, II and III, which was similar to the responses seen in normal persons negative skin lest). However, the PBMC from all the three groups of people showed comparable esponse o the T cell mitogen, phytohaemagglutinin PHA) and variable but significant responses o a crude extract of the grass pollen. While Lol p II induced a moderate response n 4 ant of 10 grass allergic individuals, it did not induct any response n others over a wide range of antigen concenuations tested. However, two of these subjects gave similar responses oll p II and Ldp III at all the doses ested. While the whole Lol p 11 and LOI p III induced similar responses, their synthetic pcptides containing segments f homologous sequences ailed to induce any response. The magnitude of the T-cell responses o the three Lo1 p allergens seems o be in the following order: Lol p I > Ld p III > Lol p II. Although grass allergic humans undergoing immunotherapy have serum antibody specific for Lol p antigens, their T cells were relatively refractive to antigen-specific stimulation m. The segments f amino acid sequence omology between Lol p II and Lo/ p III may explain their cross reactivity with specific antibodies; however, such segments do not seem to harbor a complete T-cell epilopc. 236 CLINICAL AND IMMUNOLOGIC REACTIVITY OF PATIENTS SENSITIVE TO GRASS AND . . . MULTIPLE POLLENS. J,Bousauet. R.Becaando. P.Cour. I.Chanal. F.B.MicheL Montpellier, France & FJorstel, Germany. The heterogeneity of pollen allergic individuals is well known but poorly characterized. 26 patients (25.4f10.4 yr) were studied to characterize their immunological and clinical patterns. The diagnosis of allergy was made by skin tests to pollen extracts and RAST. All relevant pollen allergens of the area were tested. The IgE response was assessed y titration of serum total IgE (PRIST), orchard grass specific IgE (RAST) and IgE- immunoblots to orchard grass pollens. Clinical reactivity was measured by nasal challenge with orchard grass pollens @-fold increment) before the pollen season and nasal symptom-medication scores between April 1 and June 15. Pollen counts were obtained during this period of survey. 13 patients were allergic only to grass pollens and 13 others allergic to grass and other pollen species including Cupressaceae, plane tree, olive and P arieturiu.. Polysen- sitized patients had significantly increased evels of serum total (~~0.05) and orchard grass specific IgE (p&05) and a greater heterogeneity of orchard grass IgE-immunoblots suggesting an enhanced qualitative and quantitative IgE- immune response to grass pollens. Nasal challenges were similar in both groups (grass pollen group: 1419i2205 grains, multiple pollen group: 973f1666 grains) and the severity of nasal symptoms during the season was not significantly different in both groups (m rtsd: grass pollen group: 9.5f5.5, multiple pollen group: 10.2f4.1). These results confirrm the heterogeneity of the IgE immune response n pollen allergic patients and suggest hat the intensity of symptoms is not related to the sensitization nor to the IgE immune response of the subjects.
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